High-fidelity telomerase activity assay based on light-triggered nucleic acid separation system for the diagnosis of bladder cancer.

In conventional methods for telomerase activity assay, the obtained telomerase sample contains a large amount of impurity, which seriously affect the accuracy of the assay. Herein, we propose a light-triggered nucleic acid separation strategy to realize high fidelity Telomerase activity assay (Lit-Telo). In the light-triggered nucleic acid separation system, a 5' terminal biotinylated and photo-cleavable (PC) linker-functionalized telomerase substrate probe (Bio-PCTS) is designed.

Genome-wide analysis of KCS genes in tomato and functional characterization of SlKCS8 and SlKCS10 in drought tolerance.

KCS, an endogenous cellular enzyme, catalyzes the elongation of fatty acid chains and plays a crucial role in the biosynthesis of plant epidermal wax. Through processes such as transfer, decarboxylation, and fixation, it contributes to plant growth and adaptation to abiotic and biotic stresses. However, the mechanism by which KCS genes participate in the response of tomato plants to driught remains unclear.

Assessing food vendor receptiveness to healthier options: a study of knowledge, attitudes, practices, and perceived barriers regarding salt, sugar, and oil reduction in cafeteria food vendors.

Background: Prioritizing policies that address major non-communicable disease (NCD) risk factors, such as poor diet, is a cost-effective way to improve health outcomes. While education can enhance knowledge and practices in the short term, recent trends show that structural and policy-based initiatives, such as setting mandatory salt, sugar, and oil (SOS) targets are effective means of changing dietary behaviours and reducing NCD risk factors. However, few studies have surveyed the KAP of food vendors in workplaces and educational institutions and assessed their receptiveness to serving low-SOS dishes.

Dissecting the stress granule RNA world: dynamics, strategies and data.

Stress granules (SGs) are cytoplasmic ribonucleoprotein granules that commonly nucleate from the interaction of translationally stalled mRNAs and RNA binding proteins. SGs are involved in the cellular adaptation to stress conditions participating in the regulation of gene expression and cell signaling. While dysregulation of SG dynamics has been increasingly implicated in human disease, a comprehensive understanding of SG composition, particularly of the RNA component, across various conditions remains elusive.

Life course socioeconomic position and cognitive aging in later life: A scoping review.

Background And Objectives: Low socioeconomic position (SEP) throughout the life course is related to poorer cognitive health in later life, but debate ensues on the life course models for this association. To advance inquiry on the topic, we conducted a scoping review.

Research Design And Methods: We examined the association between life course SEP and cognitive function in later life in observational studies-considering cognition both as a cross-sectional level and as a longitudinal trajectory across cognitive domains-and assessed whether the empirical evidence supported life course models.