Agrobacterium-mediated transient transformation of Flaveria bidentis leaves: a novel method to examine the evolution of C photosynthesis.

The genus Flaveria has been studied extensively as a model for the evolution of C photosynthesis. Thus far, molecular analyses in this genus have been limited due to a dearth of genomic information and the lack of a rapid and efficient transformation protocol. Since their development, Agrobacterium-mediated transient transformation protocols have been instrumental in understanding many biological processes in a range of plant species.

Phenylobacterium ferrooxidans sp. nov., isolated from a sub-surface geothermal aquifer in Iceland.

A novel bacterial strain, HK31-G, was isolated from a subsurface geothermal aquifer (Hellisheidi, SW-Iceland) and was characterized using a polyphasic taxonomic approach. Phylogenetic analysis of 16S rRNA gene along with phylogenomic position indicated that the novel strain belongs to the genus Phenylobacterium. Cells are motile Gram-negative thin rods.

The relationship between the youth-led Fridays for Future climate movement and voting, politician and media behaviour in Germany.

We study the relationship between the Fridays for Future climate protest movement in Germany and citizen political behaviour. In 2019, crowds of young protesters, mostly under voting age, demanded immediate climate action. Exploiting cell-phone-based mobility data and hand-collected information on nearly 4,000 climate protests, we created a highly disaggregated measure of protest participation.

Evolutionary diversification of C2 photosynthesis in the grass genus Homolepis (Arthropogoninae).

Background And Aims: To better understand C4 evolution in monocots, we characterized C3-C4 intermediate phenotypes in the grass genus Homolepis (subtribe Arthropogoninae).

Methods: Carbon isotope ratio (δ13C), leaf gas exchange, mesophyll (M) to bundle sheath (BS) tissue characteristics, organelle size and numbers in M and BS tissue, and tissue distribution of the P-subunit of glycine decarboxylase (GLDP) were determined for five Homolepis species and the C4 grass Mesosetum loliiforme from a phylogenetic sister clade. We generated a transcriptome-based phylogeny for Homolepis and Mesosetum species to interpret physiological and anatomical patterns in an evolutionary context, and to test for hybridization.

Mortality in adult patients with chronic spontaneous urticaria: A real world cohort study.

Background: Chronic spontaneous urticaria (CSU), a common and debilitating disease, is widely held not to be life-limiting, but the mortality of CSU has not been investigated.

Objective: To assess all-cause mortality in CSU patients, risk for comorbidities that are leading causes of death and impact of guideline-recommended urticaria treatments on mortality rates.

Methods: This is a retrospective population-based cohort study of electronic health records of 272,190 adult CSU patients and 12,728,913 non-urticaria controls from the US Collaborative TriNetx Analytics Network.