Stepwise virus assembly in the cell nucleus revealed by spatiotemporal click chemistry of DNA replication.

Biomolecular assemblies are fundamental to life and viral disease. The spatiotemporal coordination of viral replication and assembly is largely unknown. Here, we developed a dual-color click chemistry procedure for imaging adenovirus DNA (vDNA) replication in the cell nucleus.

Managing Growth Deceleration Associated With Attention-Deficit/Hyperactivity Disorder and Stimulant-Induced Appetite Suppression.

Attention-deficit/hyperactivity disorder (ADHD) has been diagnosed in 9.8% of children ages 3 to 17 years in the United States according to Centers for Disease Control and Prevention data from 2016 to 2019, and 62% of children with ADHD take medication as part of their treatment. Due to their well-established safety profile and high efficacy, central nervous system stimulants are used as first-line treatment for symptom reduction in children with ADHD.

DNA templated Click Chemistry via 5-vinyl-2'-deoxyuridine and an acridine-tetrazine conjugate induces DNA damage and apoptosis in cancer cells.

Aims: Click Chemistry is providing valuable tools to biomedical research, but its direct use in therapies remains nearly unexplored. For cancer treatment, nucleoside analogues (NA) such as 5-vinyl-2'-deoxyuridine (VdU) can be metabolically incorporated into cancer cell DNA and subsequently "clicked" to form a toxic product. The inverse electron-demand Diels-Alder (IEDDA) reaction between VdU and an acridine-tetrazine conjugate (PINK) has previously been used to label cell nuclei of cultured cells.

Development and validation of the German translation of the views on inpatient care (VOICE-DE) outcome measure to assess service users' perceptions of inpatient psychiatric care.

Background: Service user satisfaction in inpatient psychiatric care is often measured with instruments that have been designed by professionals, without involving the perspective of service users. Views on Inpatient Care (VOICE), developed in England, is among the first service-user-generated outcome measures which included service users' perspectives in the process.

Aims: In this study, we aimed to validate a German version of VOICE.

Active Uptake and Trafficking of Nucleoside Triphosphates .

Modified nucleoside triphosphates (NTPs) are powerful probes and medicines, but their anionic character impedes membrane permeability. As such, invasive delivery techniques, transport carriers, or prodrug strategies are required for their use. Here, we present a fluorescent 2'-deoxyribonucleoside triphosphate "TAMRA-dATP" that exhibits surprisingly high bioavailability .