Mycobacterium avium complex infection in mice: lack of exacerbation after LP-BM5 murine leukemia virus infection.

The murine leukemia virus LP-BM5 has been used to reproduce the model of murine AIDS in order to evaluate the course of infection with the MO-1 strain of Mycobacterium avium complex (MAC). LP-BM5 was inoculated in C57BL/6 mice by intravenous (i.v.

Correlation of CD8 lymphocyte activation with cellular viremia and plasma HIV RNA levels in asymptomatic patients infected by human immunodeficiency virus type 1.

The relationship between CD8 lymphocyte phenotypic alterations and virological parameters was studied in 47 asymptomatic subjects with human immunodeficiency virus type 1 (HIV-1) infection and CD4 T cell counts above 400/microliters. CD8 subsets were examined by means of three-color flow cytometry, using an extensive panel of monoclonal antibody combinations. Virological parameters were measured by both end-point dilution culture of peripheral blood mononuclear cells (PBMCs) and plasma and branched-DNA (bDNA) signal amplification of plasma HIV RNA.

Interactions between murine AIDS (MAIDS) and toxoplasmosis in co-infected mice.

We coinfected C57B1/6 mice with LP-BM5 murine leukaemia viruses, responsible for murine AIDS (MAIDS), and an avirulent strain of Toxoplasma gondii. Virus-infected mice were infected perorally on day 30 with 10 cysts of T. gondii, and T.

Toxoplasma gondii: kinetics of lymphocyte subsets in blood and spleen of perorally infected mice.

The blood and spleen lymphocyte subsets and parasite burdens in blood, lungs, and brain were determined serially in C57B1/6 mice infected with an avirulent strain of Toxoplasma gondii. Five mice were sacrificed at various time points after infection; Thy1-2+, Ly5+, CD4+, CD8+, and Ly6c+ lymphocyte subsets and macrophages were determined in blood and spleen by cytofluorometry and parasite burdens were quantified in blood, lungs, and brain by means of a tissue culture method. In infected mice, a large increase in the percentage of CD8+ lymphocytes in blood was observed from Day 14, peaking at Day 21; the percentage of CD4+ lymphocytes was not significantly modified compared with uninfected controls.

Prognostic value of phenotypic alterations in blood lymphocyte subsets in a murine retrovirus-induced immunodeficiency syndrome (MAIDS).

Mice infected with the Duplan strain of murine leukaemia virus (Dup MuLV), a retrovirus, develop a syndrome sharing several features with AIDS, including lymphadenopathy and profound immunodeficiency. We measured the changes in peripheral blood lymphocyte populations and evaluated their predictive value for the outcome of disease in C57Bl/6 mice. Animals were inoculated with Dup MuLV (SC1/Dup MuLV confluent fibroblast supernatant or spleen extract from an infected mouse).