A Comparison of Molecular Techniques for Improving the Methodology in the Laboratory of Pharmacogenetics.

One of the most critical goals in healthcare is safe and effective drug therapy, which is directly related to an individual's response to treatment. Precision medicine can improve drug safety in many scenarios, including polypharmacy, and it requires the development of new genetic characterization methods. In this report, we use real-time PCR, microarray techniques, and mass spectrometry (MALDI-TOF), which allows us to compare them and identify the potential benefits of technological improvements, leading to better quality medical care.

In-band pumped, Q-switched thulium-doped fiber laser system delivering 140 W and 7 mJ pulse energy.

We report on a highly efficient, in-band pumped, Q-switched, Tm-doped, rod-type master oscillator power amplifier (MOPA) system delivering up to 140 W average output power and 7 mJ pulse energy with a slope efficiency of 77% at 20 kHz repetition rate. The amplifier is pumped with Raman-shifted fiber lasers centered at 1692 nm. This in-band pump scheme for Tm-doped fiber lasers can significantly mitigate their quantum defect-related heat load limitations.

A global perspective on the status of clinical metabolomics in laboratory medicine - a survey by the IFCC metabolomics working group.

Objectives: Metabolomics aims for comprehensive characterization and measurement of small molecule metabolites (<1700 Da) in complex biological matrices. This study sought to assess the current understanding and usage of metabolomics in laboratory medicine globally and evaluate the perception of its promise and future implementation.

Methods: A survey was conducted by the IFCC metabolomics working group that queried 400 professionals from 79 countries.

Shotgun metagenomics and systemic targeted metabolomics highlight indole-3-propionic acid as a protective gut microbial metabolite against influenza infection.

The gut-to-lung axis is critical during respiratory infections, including influenza A virus (IAV) infection. In the present study, we used high-resolution shotgun metagenomics and targeted metabolomic analysis to characterize influenza-associated changes in the composition and metabolism of the mouse gut microbiota. We observed several taxonomic-level changes on day (D)7 post-infection, including a marked reduction in the abundance of members of the and families, and an increase in the abundance of .