Morphology and anionic polymer content in the cell wall of a glycerol-requiring mutant of Bacillus subtilis.

A glycerol-requiring mutant of Bacillus subtilis formed irregular spheres and showed disturbed septum formation, when subjected to growth limitation by the supply of glycerol. Under phosphate limitation the cells were also round and developed asymmetric septa. In magnesium-limited cultures the cells contained a thickened wall, as compared with that of the parent strain grown under the same conditions.