Identification of critical process parameters for expansion of clinical grade human Wharton's jelly-derived mesenchymal stromal cells in stirred-tank bioreactors.

Cell therapies based on multipotent mesenchymal stromal cells (MSCs) are traditionally produced using 2D culture systems and platelet lysate- or serum-containing media (SCM). Although cost-effective for single-dose autologous treatments, this approach is not suitable for larger scale manufacturing (e.g.

Efficient extracellular vesicles freeze-dry method for direct formulations preparation and use.

Despite the great knowledge achieved in the field of extracellular vesicles (EVs), the short lifetime of EVs liquid formulation still hampers the transfer of EVs technology to clinical applications. In this context, freeze-dried EVs would be advantageous thanks to the enhanced stability of solid formulations. Although some previous attempts have already been reported, the efficiency of EVs lyophilization methodologies used remains insufficient, and the characterization of the resulting EVs is still incomplete.

Acute Kidney Injury and Hyponatremia in Ultra-Trail Racing: A Systematic Review.

Background and objectives: Ultra-trail races can cause episodes of acute kidney injury (AKI) and exercise-associated hyponatremia (EAH) in healthy subjects without previous renal pathology. This systematic review aims to review the incidence of these two syndromes together and separately taking into account the length and elevation of the ultra-trail race examined. Materials and Methods: A systematic review was conducted through electronic search in four electronic databases (PubMed, EBSCO, Web of Science and Alcorze).

Bone Turnover Alterations after Completing a Multistage Ultra-Trail: A Case Study.

A series of case studies aimed to assess bone and stress fractures in a 768-km ultra-trail race for 11 days. Four nonprofessional male athletes completed the event without diagnosing any stress fracture. Bone turnover markers (osteocalcin (OC), serum C-terminal cross-linking telopeptide of type I collagen (CTX), bone-specific alkaline phosphatase (BALP), and serum turnover calcium (Ca)) were assessed before (pre) and after the race (post) and on days two and nine during the recovery period (rec2 and rec9), respectively.