Publications by authors named "M Le Gal"
Article Synopsis
- - The Wales COVID-19 Evidence Centre (WCEC) was created from 2021-23 to provide timely research evidence to inform health and social care decisions amidst the fast-paced challenges of the pandemic.
- - The WCEC employed flexible knowledge mobilisation methods, including stakeholder co-production and public engagement, to ensure that relevant findings reached the right decision-makers effectively.
- - Results showed that WCEC's processes successfully facilitated the use of rapid evidence reviews, although realizing public benefits from this work will require additional time and resources.
In this study, a phytochemical investigation on the methanol extract of Potentilla chinensis led to the isolation of eleven triterpenoids including ursolic acid (1), pomolic acid (2), tormentic acid (3), 2-epi-corosolic acid (4), 3-epi-corosolic acid (ECA, 5), 3β-hydroxyurs-11-en-13β(28)-olide (6), euscaphic acid (7), 2-epi-tormentic acid (8), corosolic acid (9), uvaol (10), and 3-O-acetylpomolic acid (11). Among them, ECA (5) showed potential anti-osteoclastogenic activity. To the best of our knowledge, this represents the first isolation of ECA (5) from P.
View Article and Find Full Text PDFUbiquitin (Ub) signals are recognized and decoded into cellular responses by Ub-receptors, proteins that tether the Ub-binding domain(s) (UBDs) with response elements. Typically, UBDs bind mono-Ub in highly dynamic and weak affinity manners, presenting challenges in identifying and characterizing their binding interfaces. Here, we report the development of a new approach to facilitate the detection of these weak interactions using split-reporter systems where two interacting proteins are proximally co-translated from a single mRNA.
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- - Cysteine is crucial for making glutathione, which protects plants from stress, and methionine, important for cell growth and protein synthesis.
- - Under oxidative stress, glutathione competes with methionine for cysteine, with elevated oxidized glutathione (GSSG) levels suppressing methionine production by accelerating the breakdown of a key enzyme involved in its synthesis.
- - This study uncovers a feedback loop where glutathione promotes cysteine’s use for stress protection at the expense of methionine production, revealing a complex relationship affecting plant growth and nutritional quality under stress.
The rapid and accurate detection of SARS-CoV-2, particularly its spike receptor-binding domain (S-RBD), was crucial for managing the COVID-19 pandemic. This study presents the development and optimization of two types of aptasensors: quartz crystal microbalance (QCM) and electrochemical sensors, both employing thiol-modified DNA aptamers for S-RBD detection. The QCM aptasensor demonstrated exceptional sensitivity, achieved by optimizing aptamer concentration, buffer composition, and pre-treatment conditions, with a limit of detection (LOD) of 0.
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