Optogenetic manipulation of nuclear Dorsal reveals temporal requirements and consequences for transcription.

Morphogen gradients convey essential spatial information during tissue patterning. While both concentration and timing of morphogen exposure are crucial, how cells interpret these graded inputs remains challenging to address. We employed an optogenetic system to acutely and reversibly modulate the nuclear concentration of the morphogen Dorsal (DL), homologue of NF-κB, which orchestrates dorso-ventral patterning in the embryo.

Identifying Markov Chain Models from Time-to-Event Data: An Algebraic Approach.

Many biological and medical questions can be modeled using time-to-event data in finite-state Markov chains, with the phase-type distribution describing intervals between events. We solve the inverse problem: given a phase-type distribution, can we identify the transition rate parameters of the underlying Markov chain? For a specific class of solvable Markov models, we show this problem has a unique solution up to finite symmetry transformations, and we outline a recursive method for computing symbolic solutions for these models across any number of states. Using the Thomas decomposition technique from computer algebra, we further provide symbolic solutions for any model.

Boosting the toolbox for live imaging of translation.

Live imaging of translation based on tag recognition by a single-chain antibody is a powerful technique to assess translation regulation in living cells. However, this approach is challenging and requires optimization in terms of expression level and detection sensitivity of the system, especially in a multicellular organism. Here, we improved existing fluorescent tools and developed new ones to image and quantify nascent translation in the living embryo and in mammalian cells.

Vision-Based UAV Detection and Localization to Indoor Positioning System.

In recent years, the technological landscape has undergone a profound metamorphosis catalyzed by the widespread integration of drones across diverse sectors. Essential to the drone manufacturing process is comprehensive testing, typically conducted in controlled laboratory settings to uphold safety and privacy standards. However, a formidable challenge emerges due to the inherent limitations of GPS signals within indoor environments, posing a threat to the accuracy of drone positioning.

Direct observation of translational activation by a ribonucleoprotein granule.

Biomolecular condensates organize biochemical processes at the subcellular level and can provide spatiotemporal regulation within a cell. Among these, ribonucleoprotein (RNP) granules are storage hubs for translationally repressed mRNA. Whether RNP granules can also activate translation and how this could be achieved remains unclear.