Publications by authors named "M L Dreyer Gillette"
Organoids are multicellular structures formed from populations of individual cells allowing modeling of structural and functional aspects of organs and tissues in normal and diseased states. They offer unique opportunities to model and treat disease. Using a mouse embryonic stem cell line, we have cultured organoids that express markers of spinal cord motor neurons as well as motor neurons found within the peripheral nervous system.
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- The study presents an optimized workflow for analyzing formalin-fixed, paraffin-embedded (FFPE) patient tissues to uncover molecular insights linked to clinical outcomes, utilizing advanced techniques like Adaptive Focused Acoustics (AFA) and liquid chromatography-tandem mass spectrometry (LC-MS/MS).
- The method allows for the analysis of up to 96 samples, identifying between 8,000-10,000 unique proteins with a high level of quantitative accuracy (<20% median CVs).
- Applied to lung adenocarcinoma FFPE blocks, the workflow demonstrates superior deep proteome coverage and efficiency, significantly contributing to biomarker discovery and proteomic research in archived samples.
Myokines and exosomes, originating from skeletal muscle, are shown to play a significant role in maintaining brain homeostasis. While exercise has been reported to promote muscle secretion, little is known about the effects of neuronal innervation and activity on the yield and molecular composition of biologically active molecules from muscle. As neuromuscular diseases and disabilities associated with denervation impact muscle metabolism, we hypothesize that neuronal innervation and firing may play a pivotal role in regulating secretion activities of skeletal muscles.
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- Despite the notable advancements in immunotherapy for cancer, only a small percentage (less than 20%) show lasting responses to immune checkpoint blockade, leading researchers to consider combination therapies that target multiple immune evasion strategies.
- Researchers analyzed data from over 1,000 tumors across ten cancers to identify seven distinct immune subtypes, examining their unique genomic, epigenetic, transcriptomic, and proteomic characteristics.
- By investigating kinase activities linked to these immune subtypes, the study uncovered potential therapeutic targets that could improve future immunotherapy approaches and precision medicine.
Optimal imaging strategies remain underdeveloped to maximize information for fluorescence microscopy while minimizing the harm to fragile living systems. Taking hint from the supercontinuum generation in ultrafast laser physics, we generated supercontinuum fluorescence from untreated unlabeled live samples before nonlinear photodamage onset. Our imaging achieved high-content cell phenotyping and tissue histology, identified bovine embryo polarization, quantified aging-related stress across cell types and species, demystified embryogenesis before and after implantation, sensed drug cytotoxicity in real-time, scanned brain area for targeted patching, optimized machine learning to track small moving organisms, induced two-photon phototropism of leaf chloroplasts under two-photon photosynthesis, unraveled microscopic origin of autumn colors, and interrogated intestinal microbiome.
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