Hyperglycemia affects neuronal differentiation and Nestin, FOXO1, and LMO3 mRNA expression of human Wharton's jelly mesenchymal stem cells of children from diabetic mothers.

Pregestational Diabetes Mellitus (PDM) during pregnancy constitutes an unfavorable embryonic and fetal development environment, with a high incidence of congenital malformations (CM). Neural tube defects are the second most common type of CM in children of diabetic mothers (CDM), who also have an elevated risk of developing neurodevelopmental disorders. The mechanisms that lead to these neuronal disorders in CDM are not yet fully understood.

Implementation of a tunable t-CRISPRi system for gene regulation in Giardia duodenalis.

Giardia duodenalis, is a binuclear and microaerophilic protozoan that causes giardiasis. Up to date, several molecular approaches have been taken to understand the molecular mechanisms of diverse cellular processes in this parasitic protozoan. However, the role of many genes involved in these processes needs further analysis.

Giardia duodenalis carries out canonical homologous recombination and single-strand annealing.

In the past decades, the ability of Giardia duodenalis to perform homologous recombination has been suggested, supported by the observations of genomic integration of foreign plasmids and the disruption of genes using CRISPR technology. Unfortunately, the direct study of a HR mechanism has not been addressed, which would be pertinent in a minimalist organism lacking fundamental DNA-repair elements and even complete pathways. In addition, the constant ploidy changes through the life cycle of this parasite highlight the conservation and relevance of homologous recombination in maintaining genomic stability.

Sirtuin GdSir2.4 participates in the regulation of rRNA transcription in the Giardia duodenalis parasite.

Giardia duodenalis is a parasite of great medical interest due to the number of infections it causes worldwide each year. Although research on epigenetic mechanisms in this protist has only begun recently, epigenetic regulation has already been shown to have important roles in encystation, antigenic variation, and resistance to antibiotics in Giardia. In this work, we show that a Giardia ortholog of Sir2, GdSir2.

Ribosomal DNA in the protozoan parasite Giardia duodenalis has a differential chromatin distribution and epigenetic markings across the subunits.

Giardia duodenalis is a parasite that causes a large number of diarrheal diseases around the world. It is noteworthy that in a large number of processes, Giardia requires fewer components than other eukaryotes, even without some organelles such as mitochondria and peroxisomes. Despite this, core histones are known to exist in Giardia and epigenetic marks have been found on them, suggesting that they somehow control the expression of certain genes.