Acoustical characterization of a portable pneumatic infrasound source.

A portable infrasound source based on a pneumatic siren design is described. The source is capable of producing narrowband tone bursts over a frequency range of approximately 0.25-10 Hz.

Acoustic radiation from an infrasonic ball-valve siren.

The concept of a ball-valve siren is developed through experimentation and theoretical modeling. The ball-valve siren is a source transducer developed for the purpose of establishing the concept of infrasound generation through the modulation of compressed air flowing through a rotating ball valve and released into the atmosphere, in the context of a siren. Directivity, frequency response, and propagation experiments were performed for the fundamental frequency component, and the results compare favorably to an empirical model based on monopole and dipole radiation.

Sulfur oxidation to sulfate coupled with electron transfer to electrodes by Desulfuromonas strain TZ1.

Microbial oxidation of elemental sulfur with an electrode serving as the electron acceptor is of interest because this may play an important role in the recovery of electrons from sulfidic wastes and for current production in marine benthic microbial fuel cells. Enrichments initiated with a marine sediment inoculum, with elemental sulfur as the electron donor and a positively poised (+300 mV versus Ag/AgCl) anode as the electron acceptor, yielded an anode biofilm with a diversity of micro-organisms, including Thiobacillus, Sulfurimonas, Pseudomonas, Clostridium and Desulfuromonas species. Further enrichment of the anode biofilm inoculum in medium with elemental sulfur as the electron donor and Fe(III) oxide as the electron acceptor, followed by isolation in solidified sulfur/Fe(III) medium yielded a strain of Desulfuromonas, designated strain TZ1.

Molecular analysis of the in situ growth rates of subsurface Geobacter species.

Molecular tools that can provide an estimate of the in situ growth rate of Geobacter species could improve understanding of dissimilatory metal reduction in a diversity of environments. Whole-genome microarray analyses of a subsurface isolate of Geobacter uraniireducens, grown under a variety of conditions, identified a number of genes that are differentially expressed at different specific growth rates. Expression of two genes encoding ribosomal proteins, rpsC and rplL, was further evaluated with quantitative reverse transcription-PCR (qRT-PCR) in cells with doubling times ranging from 6.