Vaporized hydrogen peroxide uptake by tubing used for aseptic Fill-Finish manufacturing of biopharmaceutical drug products.

Aseptic filling of biopharmaceutical products requires a grade A cleanroom environment, preferably ensured by isolators in grade C surroundings. Isolators are decontaminated before the start of filling processes using vaporized hydrogen peroxide (VHP) and filling starts at pre-defined residual VHP levels (e.g.

Facing the Dilemma of Forced Termination: What to Consider and Address When Removing a Member from a Group.

The authors explore the group leader's considerations before removing a group member from group sessions. The authors integrate an approach that warns against a too hasty decision without considering the group-as-a-whole dynamics and the possibility of scapegoating, with the approach that looks at the group therapist's countertransference that prevents them from considering the group's best interest. The rule of thumb the authors suggest is that when a severe breach of the group agreement happens, especially continuously, and when it cannot be followed by exploration, reflection, and an agreement by the group member who violated the agreement to make an effort to avoid repeating this event, the group leader might consider removing the person from the group.

Classifying covalent protein binders by their targeted binding site.

Covalent protein targeting represents a powerful tool for protein characterization, identification, and activity modulation. The safety of covalent therapeutics was questioned for many years due to the possibility of off-target binding and subsequent potential toxicity. Researchers have recently, however, demonstrated many covalent binders as safe, potent, and long-acting therapeutics.

Deep phosphoproteomics of Klebsiella pneumoniae reveals HipA-mediated tolerance to ciprofloxacin.

Klebsiella pneumoniae belongs to the group of bacterial pathogens causing the majority of antibiotic-resistant nosocomial infections worldwide; however, the molecular mechanisms underlying post-translational regulation of its physiology are poorly understood. Here we perform a comprehensive analysis of Klebsiella phosphoproteome, focusing on HipA, a Ser/Thr kinase involved in antibiotic tolerance in Escherichia coli. We show that overproduced K.