Publications by authors named "M Kulpa"
Onchocerca is an important genus of vector-borne filarial nematodes that infect both humans and animals worldwide. Many Onchocerca spp., most of medical and veterinary health relevance, are the focus of a variety of diagnostic and molecular research.
View Article and Find Full Text PDFValidation of a species-specific probe-based qPCR for detection of (Filarioidea: Onchocercidae) in Alaskan moose ().
Int J Parasitol Parasites Wildl
December 2024
Article Synopsis
- - This study explored the impact of filarioid nematodes on northern ungulates, specifically through the detection of their microfilariae in moose blood samples using a new qPCR method.
- - Researchers collected 166 blood samples from wild moose in Alaska and compared findings from the modified Knott's test and the new qPCR for detecting two types of parasites, achieving a high correlation between the two methods.
- - The qPCR method showed a detection limit of 0.157 pg/μL of DNA and provided quicker, less labor-intensive results for monitoring these parasites in moose populations.
Objectives: Assessment of anxiety-depressive disorders in an oncologically ill patient may help in the treatment process and planning psycho-oncological care. The aim of the study was to assess the level of anxiety and depression and selected psychosocial variables in cancer patients.
Methods: The study group consisted of cancer patients eligible for causal treatment for at least 6 weeks.
Article Synopsis
- Stephanofilaria stilesi is a nematode affecting cattle in North America, transmitted by horn flies, but its lesions and diagnostic methods have not been well studied.
- The study involved analyzing skin samples from 22 cattle, using methods like histology and DNA sequencing to investigate the characteristics of the lesions caused by S. stilesi.
- Findings revealed various skin lesions, with some showing the presence of adult nematodes and larvae, and confirmed S. stilesi DNA in a portion of cases, highlighting the need for better understanding of this infection.*
infection in a captive Samar cobra () in the United States: An imported case?
Int J Parasitol Parasites Wildl
April 2023
We report a case of infection in a Samar cobra () imported from the Philippines, belonging to a zoological collection in the southern United States. Under a poor post-surgical prognosis, the snake was euthanized, and at necropsy plerocercoids of a Diphyllobotriidea were found in its subcutaneous tissues and musculature. Molecular and phylogenetic analyses of the complete cytochrome oxidase c subunit I () gene of the mitochondrial DNA confirmed that the isolate belonged to the genus and was closely related to isolates from Asian countries (bootstrap support = 99.
View Article and Find Full Text PDF