Publications by authors named "M Krysiak"
Article Synopsis
- The phosphorylation of the Light-Harvesting Complex of photosystem II (LHCII) by STN7 kinase is crucial for regulating photosynthesis, particularly under changing light conditions, and can also happen in the dark due to chilling.
- Research on runner bean showed that dark-chilling leads to light-independent LHCII phosphorylation, linked to reduced plastoquinone (PQ) pool levels and potential relocation within the thylakoid membrane.
- The study suggests a new pathway where the activation of oxidative pentose phosphate pathway enzymes triggers a series of biochemical events that ultimately lead to LHCII phosphorylation, highlighting the intricate functioning of photosynthetic processes even without light.
Objectives: To ensure accurate diagnosis of infectious diseases, preanalytical factors should be considered when assessing specimen quality and subsequent test result. Accordingly, we aimed to systematically assess the effect of storage time, temperature and buffer on the analytical sensitivity of detecting the sexually transmitted pathogen, across multiple molecular diagnostic platforms.
Methods: Cultured was spiked into generic and commercial storage buffers and stored at four temperatures and five time points, ranging from -20°C to 37°C, over 30 days.
Background: The 2022 outbreak of mpox (formerly known as monkeypox) led to the spread of monkeypox virus (MPXV) in over 110 countries, demanding effective disease management and surveillance. As current diagnostics rely largely on centralised laboratory testing, our objective was to develop a simple rapid point-of-care assay to detect MPXV in clinical samples using isothermal amplification coupled with CRISPR and CRISPR-associated protein (Cas) technology.
Methods: In this proof-of-concept study, we developed a portable isothermal amplification CRISPR-Cas12a-based assay for the detection of MPXV.
Improved treatment and prevention strategies, such as antimicrobial mouthwashes, may be important for addressing the public health threat of antimicrobial-resistant Neisseria gonorrhoeae. Here, we describe the activity of seven common antibacterial mouthwashes and antiseptics against N. gonorrhoeae isolates, incorporating the use of a human saliva test matrix.
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