Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.

Monoclonal antibodies (K3-2F2 and K3-4C8) raised against hepatitis A virus were used to develop a solid-phase radioimmunoassay and enzyme-linked immunosorbent assay for the detection of hepatitis A virus and antibody. Assays with this pair of monoclonal antibodies were compared in parallel with similarly constructed solid-phase radioimmunoassays and enzyme-linked immunosorbent assays in which human polyclonal serum was used. The monoclonal antibody assay proved to be more sensitive for the detection of hepatitis A virus from fecal specimens as well as for anti-hepatitis A virus immunoglobulin G (IgG) and IgM in sera.

Monoclonal antibodies against hepatitis A virus.

Three monoclonal antibodies (K2-4F2, K3-2F2, and K3-4C8) of the immunoglobulin G2a class were raised against hepatitis A virus. The specificity of these antibodies was confirmed by immune electron microscopy, solid-phase radioimmunoassay, and in vitro neutralization in cell culture. Binding studies suggested that they all recognize closely related antigenic determinants.

Monoclonal hybridoma antibodies against human IgE and their use in a rapid and sensitive enzyme immunoassay for the semiquantitative assessment of total IgE levels in human blood.

A simple semiquantitative enzyme immunoassay (EIA) for the rapid estimation of IgE levels in specimens of human blood, plasma or serum is described. The test requires little labour input and does not require highly trained personnel or instrumentation. By using two monoclonal antibodies of different anti-IgE specificities it is possible, with a single incubation of 20 min at ambient temperature, to detect elevated IgE levels (greater than or equal to 333 IU/ml) within a total test time of 25 min, and low levels of IgE (less than or equal to 10 IU/ml) within 35 min.