Publications by authors named "M Kloster-Landsberg"
Living cells are heterogeneous and rapidly changing biological samples. It is thus desirable to measure molecular concentration and dynamics in many locations at the same time. In this note, we present a multi-confocal setup capable of performing simultaneous fluorescence correlation spectroscopy measurements, by focusing the spots with a spatial light modulator and acquiring data with a monolithic 32 × 32 single-photon avalanche photodiode array.
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- Heat shock factor 1 (HSF1) is crucial for the heat shock response, helping protect cells from stress by binding to and activating heat shock protein (hsp) genes and satellite III sequences.
- During stress, HSF1 forms nuclear stress bodies (nSBs) which enhance transcription sites, showcasing its role in managing cellular responses.
- The study uses advanced microscopy techniques to reveal that HSF1 interacts with DNA in two ways: through specific binding to Heat Shock Elements and satellite sequences, and through more transient interactions, with the trimerization domain being essential for low-affinity chromatin binding.
Heat shock triggers a transient and ubiquitous response, the function of which is to protect cells against stress-induced damage. The heat-shock response is controlled by a key transcription factor known as heat shock factor 1 (HSF1). We have developed a multiconfocal fluorescence correlation spectroscopy setup to measure the dynamics of HSF1 during the course of the heat-shock response.
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