Immunohistochemical evaluation of superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes of dairy cattle and farm-raised deer by a computer-assisted analysis of microscopic images.

The effectiveness of the immunohistochemical method in determining Cu/Zn SOD concentrations in red blood cells of dairy cattle and farm-raised deer was evaluated by a computer-assisted analysis of microscopic images and scanning technique. Superoxide dismutase (Cu/Zn SOD) concentrations in erythrocytes were determined in smears of whole blood samples collected from 16 Polish Holstein-Friesian cows and 22 farm-raised deer in spring. Mouse anti-bovine SOD (Cu-Zn) monoclonal antibodies (2F5, Serotec) were used in 1:50 dilution.

Serum paraoxonase-1 activity of dairy Holstein-Fresian cows in different lactation stages--preliminary study.

The objective of this study was to investigate paraoxonase-1 (PON-1) activity in different lactation stages. The study was conducted on Holstein--Friesian dairy cows in 2nd and 3rd lactation. A significant decrease in paraoxonase activity was found in the postpartum period and during peak of lactation.

The role of metal ions in biological oxidation--the past and the present.

Two theories, one based on the metabolism of inorganic substances, the other on metabolism of organic substances, have played an important role in the explanation of the origin of life. They demonstrate that the original environment of life on Earth was seawater containing micronutrients with structural, metabolic and catalytic activity. It is assumed that the first primitive organisms lived around 3.

High prevalence of Candida yeast in milk samples from cows suffering from mastitis in poland.

Mastitis is an economically important disease in which fungi belonging to the genus Candida may participate as etiological agents. This study focused on determining the frequency of fungal isolation and differentiation of fungal species isolated from milk of mastitic cows. Sixty-six milk samples from mastitic cows were studied, and 55 strains of fungi were isolated.

Concentration of bovine pregnancy associated glycoprotein in plasma and milk: its application for pregnancy diagnosis in cows.

Pregnancy diagnosis is an important part in reproduction management of ruminants. The aim of the study was to use a new method for evaluating the bPAG and cPAG in milk and blood bPAG and compare this results with the other method for pregnancy diagnosis in the cows. The study was carried out in 220 Holstein Frisian cows.