[Molecular genetic characteristics of the newcastle disease virus velogenic strains isolated in Russia, Ukraine, Kazakhstan, and Kirghizia].

The F gene fragment of 79 Newcastle disease virus (NDV) strains isolated from domestic and synanthropic birds in Kazakhstan, Kirghizia, Ukraine, and Russia in 1993 to 2007 was comparatively analyzed. Phylogenetic analysis of test isolates and reference NDV strains obtained from the GenBank was carried out by polymerase chain reaction with subsequent sequencing and comparative analysis of 154-bp nucleotide sequences in the main functional region of the F gene. All newly characterized isolates belong to three NDV genotype VII subgroups: VIIa, VIIb, VIId.

[A method of preparing antibody erythrocytic diagnostic means for detecting viruses].

It was found possible to use method for preparation of antibody erythrocytic diagnosticums for detection of agents of influenza and arboviral infections. Test systems prepared by the developed method were superior in sensitivity to immunoreagents prepared with previously proposed amidol. Testing of 1387 tick samples collected in various climatic geographical zones of the Republic of Kazakhstan detected arboviral antigens in 4.

[Yucaipa-like viruses isolated in Kazakhstan in 1987-1989].

From domestic birds 13 strains of avian paramyxoviruses, serotype 1, and 14 strains of serotype 2 were isolated. Avian paramyxoviruses, serotype 2, differ antigenically and biologically from each other and from the prototype variant chicken/Yucaipa/California/56. The virus was also detected experimentally in birds having contact with the infected specimens.

[The sensitivity of a number of cell cultures to different types of influenza viruses and their reassortants].

The study of reproductive activity of human and animal influenza A, B, and C viruses as well as influenza A virus reassortants in some cell cultures allowed one to determine the range of cells susceptible for each type (subtype) of the viruses. Differences in the range of cells were demonstrated for different strains of influenza viruses of the same antigenic subtype. It was noted that reassortants of influenza A viruses with the same hemagglutinin subtypes as the parental strains had a wider range of susceptible cell lines and a higher reproductive capacity in these cells.