Publications by authors named "M Kerfoot"
Efficient quantitative assays for measurement of viral replication and infectivity are indispensable for future endeavors to develop prophylactic or therapeutic antiviral drugs or vaccines against SARS-CoV-2. We developed a SARS-CoV-2 cell-cell transmission assay that provides a rapid and quantitative readout to assess SARS-CoV-2 spike hACE2 interaction in the absence of pseudotyped particles or live virus. We established two well-behaved stable cell lines, which demonstrated a remarkable correlation with standard cell-free viral pseudotyping for inhibition by convalescent sera, small-molecule drugs, and murine anti-spike monoclonal antibodies.
View Article and Find Full Text PDFArticle Synopsis
- Self-harm is prevalent among adolescents, and while many studies have looked into interventions, evidence supporting their effectiveness in reducing repeat incidents is scarce, especially with long-term follow-up.
- The SHIFT trial involved 832 young participants, evaluating the impact of family therapy versus standard treatment over an extended period of up to 82.5 months, focusing on repeat self-harm incidents leading to hospital visits.
- Results showed no significant difference in self-harm rates between the two treatment groups over the extended follow-up, although older adolescents (15-17 years) showed a lower self-harm rate compared to younger ones (11-14 years).
Cell penetrating peptides (CPPs) offer great potential to deliver therapeutic molecules to previously inaccessible intracellular targets. However, many CPPs are inefficient and often leave their attached cargo stranded in the cell's endosome. We report a versatile platform for the isolation of peptides delivering a wide range of cargos into the cytoplasm of cells.
View Article and Find Full Text PDFBackground: Self-harm in adolescents is common and repetition rates high. There is limited evidence of the effectiveness of interventions to reduce self-harm.
Objectives: To assess the clinical effectiveness and cost-effectiveness of family therapy (FT) compared with treatment as usual (TAU).
Background: Self-harm in adolescents is common and repetition occurs in a high proportion of these cases. Scarce evidence exists for effectiveness of interventions to reduce self-harm.
Methods: This pragmatic, multicentre, randomised, controlled trial of family therapy versus treatment as usual was done at 40 UK Child and Adolescent Mental Health Services (CAMHS) centres.