Flow cytometry-based diagnostic approach for inborn errors of immunity: experience from Algeria.

Purpose: In this study, we retrospectively reviewed the use of flow cytometry (FCM) in the diagnosis of inborn errors of immunity (IEIs) at a single center in Algeria. Sharing insights into our practical experience, we present FCM based diagnostic approaches adapted to different clinical scenarios.

Methods: Between May 2017 and February 2024, pediatric and adult patients presenting with clinical features suggestive of immunodeficiency were subjected to FCM evaluation, including lymphocyte subset analysis, detection of specific surface or intracellular proteins, and functional analysis of immune cells.

[Forty years of experience in the treatment of children with type 1 diabetes mellitus in Algeria].

Unbalanced type I diabetes mellitus in childhood can lead to metabolic and microangiopathic complications, including growth retardation, poor social integration and even death, particularly in developing countries. The author reviews the files of an infantile diabetic clinic created in 1968 with very limited human and material resources. A total of 722 children with type 1 diabetes mellitus were regularly monitored Microangiopathic complications became less frequent and occurred later in the disease course.

[Recurrent non-diabetic acido-ketotic coma. Apropos of a case].

A case of recurrent transient episodes of acido-ketosis with complete recovery in a 3-year-old girl with normal development is reported. Investigations failed to determine the cause but this case is compatible with recurrent organic acidemia.

[Evaluation of the assay of serum fructosamine in the surveillance of diabetes in children and adolescents].

Serum fructosamine and glycosylated haemoglobin were measured 4 times over 2 month period (1st, 15th, 30th and 60th days) in 40 young diabetics and in 26 controls. A positive correlation was found between these two parameters at the 15th, 30th and 60th days (0.44 less than r less than 0.

[Hydatid cyst of the pericardium and diaphragm. Apropos of a case].

A rare cas of hydatic disease is reported, located both in the pericardium and diaphragm. The pericardial cyst was diagnosed on the basis of clinical examination and echocardiography. The diaphragmatic cyst was only discovered during surgery.