Publications by authors named "M Kastelic"
Polysorbates, widely used excipients in drug formulations, present a stability challenge due to complex degradation processes. This study investigates the hydrolysis of polysorbate (PS) under temperature stress (50 °C), focusing on the impact of primary packaging materials (glass vs. plastic vials), buffers (histidine and acetic acid), counterions (chloride vs.
View Article and Find Full Text PDFIntroduction: X-linked hypophosphatemia is an orphan disease of genetic origin and multisystem involvement. It is characterized by a mutation of the PHEX gene which results in excess FGF23 production, with abnormal renal and intestinal phosphorus metabolism, hypophosphatemia and osteomalacia secondary to chronic renal excretion of phosphate. Clinical manifestations include hypophosphatemic rickets leading to growth abnormalities and osteomalacia, myopathy, bone pain and dental abscesses.
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- - RPAV was first identified in red pandas at a U.S. zoo in 2018 and was found to have a 50% infection rate in U.S. zoos, with limited studies outside the U.S.
- - In a study of 134 red pandas in European zoos, RPAV was detected in 21 out of 62 zoos (33.9%), with an estimated individual infection rate of 24.2%, notably higher in adult females compared to adult males.
- - Zoos with confirmed RPAV cases reported more instances of alopecia and general clinical signs, while other health issues did not differ; additionally, RPAV is intermittently excreted in feces, suggesting that actual prevalence
Article Synopsis
- The study compared various methods of blood and other sample collection to measure "immunoreactive" C (iC) concentrations in Alpine ibexes, revealing venipuncture yielded the highest serum values.
- Statistically significant differences in iC concentrations were noted between methods (venipuncture, kissing bugs, and leeches) and sample types (saliva, faeces, and hair), with saliva showing the lowest concentrations.
- Additionally, factors like sex and age notably influenced C concentrations, while collecting tears and urine proved labor-intensive, limiting their use in measurements.
Blood sampling is a challenging procedure in many captive animals. Although manual restraint or anesthesia are usually possible, they entail intense stress and a high risk of injuries or organ failure. Blood sampling using medicinal leeches () represents a promising non-invasive alternative to venipuncture; however, leech blood meal was to date used only for qualitative analyses such as genetic or serological screenings.
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