Doxorubicin plus interleukin-2 chemoimmunotherapy against breast cancer in mice.

As recently characterized, following s.c. implantation into syngeneic C57BL/6 mice, E0771 tumor invades locally into dermal layers and peritoneum, metastasizes to the lung, and induces a nonspecific immunosuppression in the host.

Distant metastasis from subcutaneously grown E0771 medullary breast adenocarcinoma.

Background: Breast cancer treatments are most effective when initiated early, with very poor efficacy against metastatic disease. In seeking a readily metastasizing mouse breast cancer model to facilitate the search for effective therapies, E0771 medullary adenocarcinomas implanted subcutaneously in syngeneic C57BL/6 mice were studied.

Materials And Methods: Standard pathological, histological and immunological methodologies were used.

Fluorouracil plus leucovorin induces submandibular salivary gland enlargement in rats.

The administration of 5-fluorouracil (FU) and leucovorin (LV) to rats induced a previously unreported sialoadenitis-like toxicity. Four different treatment regimens were used: daily-times-5 iv or ip injections of LV (200 mg/kg) followed 30 minutes later by FU (27.5 mg/kg or 35 mg/kg).

Immunomodulation in cancer therapeutics.

Clinical verification is being obtained, with a variety of different therapeutic approaches, for the concept that anticancer treatments based on exploiting the host's own antitumor defense mechanism can be beneficial. Nevertheless, as was seen with both chemotherapeutic and radiation treatments, the benefit of single agent treatments is not great. It is anticipated that, in attempting to realize the maximal potential of anticancer treatments based on exploiting the host's own antitumor defense mechanism, it will be necessary to utilize combination therapies.

Doxorubicin induces specific immune functions and cytokine expression in peritoneal cells.

To examine the basis of the immune modulation induced by the anticancer agent doxorubicin (DOX), the immunophenotype, tumoricidal activity, cytokine protein and mRNA expression were determined using peritoneal exudate cells (PEC) from saline-treated (untreated) and DOX-treated mice. A greater percentage of PEC from DOX-treated mice than from untreated mice were adherent to plastic, had characteristics of granulocytes, and were positive for the NK1.1, CD11b/Mac-1, and CD3 markers.