Publications by authors named "M J W Adjobo-Hermans"
Article Synopsis
- Dimeric nicotinamide nucleotide transhydrogenase (NNT) is a crucial enzyme located in the mitochondrial inner membrane, involved in converting NADP/NADH to NADPH/NAD while facilitating proton influx, but its specific roles and regulation in health and diseases like cancer are still not thoroughly understood.! -
- Research on NNT has been conducted through studies on gene mutations in specific models (like GCCD4 patients and C57BL/6J mice) and effects of NNT alterations in cancer cells, revealing both common and unique functional issues, yet information on NNT's physiological role in humans remains limited.! -
- To advance understanding of NNT's functions and effects in various conditions, future
Article Synopsis
- Mitochondrial morphology and membrane potential are key indicators of mitochondrial health, and these can be analyzed using fluorescent dyes in living cells.
- The study focuses on using TMRM and Mitotracker Green FM to assess both mitochondrial shape and membrane potential in primary human skin fibroblasts.
- An integrated protocol is provided for quantifying these parameters through epifluorescence microscopy, specifically highlighting its effectiveness with large, flat cells at high magnification.
Ndufs4 knockout mice with isolated complex I deficiency engage a futile adaptive brain response.
Melissa A E van de Wal Cenna Doornbos Janne M Bibbe Judith R Homberg Clara van Karnebeek Merel J W Adjobo-Hermans
Biochim Biophys Acta Proteins Proteom
January 2025
Article Synopsis
- Paediatric Leigh syndrome (LS) is a severe neurodegenerative disorder caused by mutations in the NDUFS4 gene, which affects mitochondrial function, and currently has no effective treatments.
- Researchers used whole-body Ndufs4 knockout (KO) mice to analyze differences in brain proteomes between these mice and wildtype mice, identifying variations across different brain regions.
- Findings highlighted reduced levels of critical mitochondrial components in specific brain areas and suggested that attempts to stimulate certain metabolic pathways could be harmful, prompting the need for new therapeutic strategies for managing LS.
Article Synopsis
- * The cytotoxicity of nHA is influenced by their physicochemical properties, such as size, shape, crystallinity, and how they cluster together (agglomeration), making it challenging to understand their safety for cells.
- * Research indicates that agglomeration increases the cytotoxicity of nHA by raising intracellular calcium levels and producing reactive oxygen species (ROS), suggesting that optimizing these properties is crucial for developing nHA for biomedical uses.
Mitochondrial membrane potential (Δψ) and morphology are considered key readouts of mitochondrial functional state. This morphofunction can be studied using fluorescent dyes ("probes") like tetramethylrhodamine methyl ester (TMRM) and Mitotrackers (MTs). Although these dyes are broadly used, information comparing their performance in mitochondrial morphology quantification and Δψ-sensitivity in the same cell model is still scarce.
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