Publications by authors named "M J Troulis"
Article Synopsis
- - The objective of the study was to analyze clear cell sarcoma (CCS) in relation to two other rare tumors: clear cell odontogenic carcinoma (CCOC) and clear cell carcinoma of the salivary gland (CCC) to find similarities among them.
- - The research involved reviewing existing literature, collecting data on the characteristics, symptoms, imaging results, and genetic features of these tumors through PubMed.
- - Findings indicated that CCS shares histological similarities and genetic profiles with CCOC and CCC, with all three tumors showing a tendency to occur in soft tissue and bones, primarily affecting women and typically presenting at different ages.
Patient-derived organoid models hold promise for advancing clinical cancer research, including diagnosis and personalized and precision medicine approaches, and cytology, in particular, plays a pivotal role in this process. These three-dimensional multicellular structures are heterogeneous, potentially maintain the cancer phenotype, and conserve the genomic, transcriptomic, and epigenomic patterns of the parental tumors. To ensure that only tumor tissue is used for organoid development, cytologic validation is necessary before initiating the process of organoid generation.
View Article and Find Full Text PDFObjective: A specific type of mesenchymal stem/progenitor cells (MSPCs), CD105 is reported to aid in cartilage regeneration through TGF-β/Smad2-signalling. The purpose of this study was to identify and characterize CD105 MSPCs in temporomandibular joint (TMJ) cartilage.
Materials And Methods: MSPCs were isolated from mouse TMJ condyle explants and evaluated for their clonogenicity and pluripotential abilities.
Chondrogenic differentiation of mouse CD105+ stem/progenitor cells on amino-group-functionalized biosilica-hydrogel scaffolds.
Oral Surg Oral Med Oral Pathol Oral Radiol
April 2023
Objectives: Mesenchymal stem/progenitor cells (MSPCs) are critical for tissue regeneration. Moreover, the CD105 antigen identifies early MSPCs with increased chondrogenic differentiation ability. We hypothesized that amine-(NH)-functionalized biosilica incorporating hydrogel scaffolds, seeded with mCoSPCs would contribute to creating tissue-engineered scaffolds, capable of de novo cartilage synthesis.
View Article and Find Full Text PDFDetection of EWSR1 fusions in CCOC by targeted RNA-seq.
Oral Surg Oral Med Oral Pathol Oral Radiol
August 2022
Objective: To describe the application of a targeted RNA sequencing assay to detect fusion transcripts in formalin-fixed paraffin-embedded (FFPE), non-decalcified samples of clear cell odontogenic carcinoma (CCOC) and related tumors, and to add to knowledge of the genetic drivers of CCOC.
Study Design: Five FFPE tissues, including intraosseous CCOC (n = 3), clear cell carcinoma of the salivary gland (CCC, n = 1), and Ewing sarcoma (ES, n = 1), were analyzed by targeted RNA-seq to detect fusions.
Results: The 3 intraosseous CCOC samples harbored EWSR1 translocations: EWSR1-ATF1 (n = 2) and EWSR1-CREM (n = 1); the CCC sample contained an EWSR1-ATF1 fusion; and the ES sample contained an EWSR1-FLI1 fusion detected by RNA-seq.