Publications by authors named "M J Rasaee"
A comparison between different chemical fractionation methods for immunoglobulin preparation.
J Immunoassay Immunochem
January 2025
Background: Application of antibodies in therapeutics and diagnostics are growing Continually. Herein, we aimed to find the most qualified immunoglobulin (Ig) chemical preparation method.
Methods: A rabbit was immunized against recombinant SARS-CoV-2 nucleocapsid (NP) and reactive polyclonal antibodies were prepared using the ammonium sulfate (AS), caprylic acid (CA), polyethylene glycol (PEG), and caprylic acid/ammonium sulfate (CA/AS) methods.
Characterization of an engineered ACE2 protein for its improved biological features and its transduction into MSCs: A novel approach to combat COVID-19 infection.
Int J Biol Macromol
October 2024
Article Synopsis
- - Transduced mesenchymal stem cells (MSCs) engineered to express ACE2 can serve as decoys to prevent COVID-19 from infecting healthy lung cells by blocking the virus's entry.
- - The study involved integrating a mutated ACE2 gene into the MSCs, and various tests confirmed that the modified ACE2 had improved interactions with the SARS-CoV-2 spike protein and better thermal stability.
- - This novel approach could enhance targeted drug delivery with fewer side effects, and the engineered MSCs might be combined with other COVID-19 treatments and adapted for other diseases.
Background: The programmed cell death protein-1 (PD-1)/programmed death receptor ligand 1 (PD-L1) axis critically facilitates cancer cells' immune evasion. Antibody therapeutics targeting the PD-1/PD-L1 axis have shown remarkable efficacy in various tumors. Immuno-positron emission tomography (ImmunoPET) imaging of PD-L1 expression may help reshape solid tumors' immunotherapy landscape.
View Article and Find Full Text PDFNoninvasive PET imaging of tumor PD-L1 expression with Cu-labeled Durvalumab.
Am J Nucl Med Mol Imaging
February 2024
Breast cancer (BrCa) ranks as the most prevalent malignant neoplasm affecting women worldwide. The expression of programmed death-ligand 1 (PD-L1) in BrCa has recently emerged as a biomarker for immunotherapy response, but traditional immunohistochemistry (IHC)-based methods are hindered by spatial and temporal heterogeneity. Noninvasive and quantitative PD-L1 imaging using appropriate radiotracers can serve to determine PD-L1 expression in tumors.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the use of human placental mesenchymal stem cell-derived exosomes (HPMSCs-Exosomes) to treat severe spinal cord injuries (SCI) in rats, focusing on improvements in motor function.
- Researchers conducted intrathecal injections of these exosomes during the acute phase of SCI and observed significant functional recovery over a six-week period compared to control groups.
- Results indicate that HPMSCs-Exosomes reduce neuron apoptosis, lower glial scar formation, and promote tissue preservation, suggesting a promising therapeutic strategy for enhancing recovery after SCI.