Relationship between dietary retinol and alpha-tocopherol and lipid peroxidation in rat liver cytosol.

The effects of retinol and alpha-tocopherol-deficient and supplemented diets on the cytosolic concentration of thiobarbituric acid reactive substances (TBARS) in rat liver have been studied. Physiological lipoperoxidation (LPO) was observed in liver cytosol of control rats (TBARS = 0.315 +/- 0.

Free malondialdehyde determination by HPLC applied to microsomal studies.

Malondialdehyde (MDA) is a product of lipid peroxidation in vivo. The most widely employed method for determination of free MDA is based on its reaction with thiobarbituric acid (TBA) which produces a pink pigment with an absorption maximum at 532-535 nm. However, quantitation of MDA is limited by its lack of specificity and a high performance liquid chromatographic (HPLC) method was recently developed in several laboratories.

Free radical inhibitor effect of retinol after carbon tetrachloride intoxication in the rat.

A study was conducted to explore the free radical inhibitor effect of retinol in Male Wistar rats. When retinol-deprived animals were considered retinol-depleted (after a period of 8 weeks), rats of each group, control and depleted, received an intraperitoneal injection of mineral oil (5 ml/kg body weight) or an equivalent volume of 20% carbon tetrachloride (CCl4) dissolved in mineral oil. The animals were killed by decapitation 4 h after administration of CCl4 and liver, heart, spleen, brain and testes were quickly removed.

Polyacrylamide gradient gel electrophoresis of cytosolic retinol- and retinoic acid-binding proteins: application to rat testis and liver.

Distribution and cellular levels of retinol-binding protein and retinoic acid-binding protein, involved in the molecular action of retinoids, were analyzed in rat testis and liver. Both binding proteins of cytosolic extracts were separated by linear-polyacrylamide gradient gel electrophoresis and following electrophoretic separation, could be visualized by complementary identification tests such as autoradiography and marker proteins. The concentration of the binding proteins were evaluated by scanning the polyacrylamide gradient gels and the resulting data were found to be in accordance with those obtained by counting radioactivities.