Co-surfactant roles of amino acids at oil-water interface: Application in low-pH emulsions to regulate physical and oxidative stabilities.

Sucrose monopalmitate (SMP) is an effective surfactant for emulsification, but exhibits poor stability in low pH environments, due to neutralized surface charges. To improve SMP-based emulsions, we used food-grade amino acids as co-surfactants. Lysine, histidine, phenylalanine, and tryptophan lowered the water-oil interfacial tension.

Spatial transcriptomics identifies novel Pseudomonas aeruginosa virulence factors.

To examine host-pathogen interactions, we leveraged a dual spatial transcriptomics approach that simultaneously captures the expression of Pseudomonas aeruginosa genes alongside the entire host transcriptome using a murine model of ocular infection. This method revealed differential pathogen- and host-specific gene expression patterns in infected corneas, which generated a unified transcriptional map of infection. By integrating these data, we developed a predictive ridge regression model trained on images from infected tissues.

Tumor-to-tumor metastasis of clear cell renal cell carcinoma to meningioma: illustrative case.

Background: Tumor-to-tumor metastasis (TTM) is an exceptionally rare phenomenon. To date, only 12 cases of the metastasis of clear cell renal cell carcinoma (ccRCC) to a meningioma have been reported in the literature. The authors present a unique case of TTM involving ccRCC metastasizing to a meningioma in a patient with a history of both primary cancers.

Mitochondria sense bacterial lactate and drive release of neutrophil extracellular traps.

Neutrophils induce oxidative stress, creating a harsh phagosomal environment. However, Staphylococcus aureus can survive these conditions, requiring neutrophils to deploy mechanisms that sense bacterial persistence. We find that staphylococcal lactate is a metabolic danger signal that triggers neutrophil extracellular trap release (NETosis).

Fungal Als proteins hijack host death effector domains to promote inflammasome signaling.

High-damaging Candida albicans strains tend to form hyphae and exacerbate intestinal inflammation in ulcerative colitis patients through IL-1β-dependent mechanisms. Fungal agglutinin-like sequence (Als) proteins worsen DSS-induced colitis in mouse models. FADD and caspase-8 are important regulators of gut homeostasis and inflammation.