Using resolution calculations to assess changes in capillary electrophoresis run parameters.

Capillary electrophoresis is widely used in the forensic community for the analysis of Short Tandem Repeat DNA. The CE system used in most forensic laboratories allows the user to modify standard operational protocols to accommodate some samples that fall outside of interpretational guidelines. We have made operational changes and monitored system resolution and the ability of the software to identify alleles as a result of these modifications.

TWGDAM validation of the AmpFlSTR Profiler Plus and AmpFlSTR COfiler STR multiplex systems using capillary electrophoresis.

Prior to forensic implementation, a profiling system requires validation following the recommendations presented by the Technical Working Group on DNA Analysis Methods (TWGDAM). In this work two such systems, AmpFlSTR Profiler Plus and AmpFfSTR COfiler have been validated according to the guidelines provided by TWGDAM. Profiler Plus and COfiler simultaneously amplify nine and six STR loci respectively; both also amplify a portion of the amelogenin gene.

Evaluation of capillary electrophoresis performance through resolution measurements.

The application of resolution measurements to an electrophoretic system can give a quantitative analysis of the health of that system. Capillary electrophoresis has become a routine method for the analysis of DNA and resolution measurements can be used to assess the resulting electropherogram. A number of methods are available to evaluate resolution and three methods are detailed in the current work.

Validation of capillary electrophoresis for analysis of the X-Y homologous amelogenin gene.

Capillary electrophoresis (CE) is a versatile technology with tremendous potential applications in forensic science. A refinement of the traditional slab-gel, separation occurs in a liquid polymer contained within a capillary. The capillary electrophoresis unit is computer-driven, semi-automating the loading and analysis of samples.

Capillary electrophoresis STR analysis: comparison to gel-based systems.

Capillary electrophoresis is a relatively new technology for the forensic analysis of STRs, and may be an excellent alternative to traditional gel-based methods. Eighty samples, obtained from six different laboratories which had previously analyzed the samples using a variety of gel-based methods, were reanalyzed by CE. It was determined that CE could obtain the same allelic designation as that reported by the comparison laboratory.