Pharmacological inhibition of the Janus Kinases enhances ASCL1 protein stability and transcriptional activity.

Background: Achaete-Scute complex homolog 1 (ASCL1) is a multi-faceted pro-neural transcription factor, playing a role in several processes during embryonic development and into adulthood, including neural progenitor proliferation and neuronal differentiation. This versatility is achieved through tightly controlled expression of ASCL1, either via integrating intracellular signalling cues or stabilisation at the protein level. The role of kinases in ASCL1-mediated neurogenesis is emerging, but to date few kinases have been attributed to act directly or indirectly on ASCL1.

Predictors of treatment failure with parasacral transcutaneous electrical nerve stimulation in children and adolescents with bladder and bowel dysfunction.

Introduction: Parasacral Transcutaneous Electrical Nerve Stimulation (parasacral TENS) has emerged as a promising treatment for children with Bladder and Bowel Dysfunction (BBD), being an efficient method without direct adverse effects. Little is known about clinical characteristics associated with poorer treatment outcomes in this specific patient group.

Objective: To evaluate potential clinical predictors of parasacral TENS treatment failure in children and adolescents with BBD.

Benchmarking open-source large language models on Portuguese Revalida multiple-choice questions.

Objective: The study aimed to evaluate the top large language models (LLMs) in validated medical knowledge tests in Portuguese.

Methods: This study compared 31 LLMs in the context of solving the national Brazilian medical examination test. The research compared the performance of 23 open-source and 8 proprietary models across 399 multiple-choice questions.

Scalable propionic acid production using Cutibacterium acnes ZW-1: Insights into substrate and pH-driven carbon flux.

An acid-resistant Cutibacterium acnes ZW-1 was isolated from human skin, and propionic acid (PA) production under different substrate and pH conditions was studied. When the molar ratio of lactic acid (LA) to acetic acid (AA) was 7:1 and the pH was 6.5, the PA concentration could reach 64.

An integrated perspective on single-cell and spatial transcriptomic signatures in high-grade gliomas.

High-grade gliomas (HGG) are incurable brain malignancies in children and adults. Breakthrough advances in transcriptomic technologies unveiled the intricate diversity of cellular states and their spatial organization within HGGs. We qualitatively integrated 55 neoplastic transcriptomic signatures described in 17 single-cell and spatial RNA sequencing-based studies.