Publications by authors named "M J Bienkowski"
Failure of central nervous system (CNS) axons to regenerate after injury results in permanent disability. Several molecular neuro-protective and neuro-regenerative strategies have been proposed as potential treatments but do not provide the directional cues needed to direct target-specific axon regeneration. Here, we demonstrate that applying an external guidance cue in the form of electric field stimulation to adult rats after optic nerve crush injury was effective at directing long-distance, target-specific retinal ganglion cell (RGC) axon regeneration to native targets in the diencephalon.
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- * The reactive thioester group in C4d allows it to label nearby surfaces, making it useful for immunohistochemical staining, though there are concerns about the reliability due to high background signals in tissues.
- * An improved rabbit anti-C4d antibody has been developed, showing less cross-reactivity and better performance in staining kidney transplant biopsies compared to existing antibodies, enhancing its diagnostic accuracy.
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- Micronuclei (MN) are formed through various mechanisms, and their frequent presence in tumors suggests they might actively contribute to cancer progression rather than being just passive byproducts.
- This study highlights how damages during cell division can lead to the accumulation of Emerin in MN, which lack Lamin A/C and feature the Lamin-B receptor and Sec61β.
- Emerin mislocalization to MN is linked to a poor prognosis in prostate cancer patients and is associated with increased migratory and invasive behaviors of tumor cells, particularly in environments rich in collagen.
Robotically assisted proteomics provides insights into the regulation of multiple proteins achieving excellent spatial resolution. However, developing an effective method for spatially resolved quantitative proteomics of formalin fixed paraffin embedded tissue (FFPE) in an accessible and economical manner remains challenging. We introduce non-robotic In-insert FFPE proteomics approach, combining glass insert FFPE tissue processing with spatial quantitative data-independent mass spectrometry (DIA).
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