An implication of novel methodology to study pancreatic acinar mitochondria under in situ conditions.

Mitochondria maintain numerous energy-consuming processes in pancreatic acinar cells, yet characteristics of pancreatic mitochondrial oxidative phosphorylation in native conditions are poorly studied. Besides, it is not known which type of solution is most adequate to preserve functions of pancreatic mitochondria in situ. Here we propose a novel experimental protocol suitable for in situ analysis of pancreatic mitochondria metabolic states.

Genes expression of calcium signaling molecules in salivary glands of Drosophila melanogaster larvae.

Genes expression of the Itp-r-83A, Ca-P60A, olf186-F which encode inositol-1,4,5-trisphosphate receptor, endoplasmic reticulum Ca(2+)-ATPase and Oral protein--component of the store-operated Ca(2+)-entry respectively, was determined in salivary glands of Drosophila melanogaster larvae. For this purpose, the method of reverse transcription-polymerase chain reaction (RT-PCR) was used. Our results suggest that mentioned Ca(2+)-transport systems play an important role in maintaining of Ca(2+)-homeostasis in larval salivary glands of Drosophila melanogaster.

[Effect of temperature and monensin on the level of intracellular sodium and pH in perfused RIF-1 cultured cells].

The direct measurement of temperature in subcutaneously (sc) implanted tumors shows that the actual tumor temperature is by 3-4 degrees C lower than the normal body temperature. Thus, the temperatures usually used for tumor hyperthermia are in fact heating the sc-tumors from 33 to 37 degrees C. The temperature increase during the perfusion of radiation-induced fibrosarcoma (RIF-1) cells from 33 to 37 degrees C caused a reversible increase in intracellular 23Na ([Na+]i) NMR signal intensity by 50-60%.

[Kinetic characteristics of Ca2+, Mg2+-ATPases in cells of the submandibular salivary gland of rats].

Kinetic properties of Ca2+, Mg2+-ATPases membranes from acinar cells of rat submandibular salivary glands have been investigated. It was found that kinetics of ATP hydrolysis dependent on Ca2+, Mg2+-ATPases corresponds to the first-order reaction during first 2 min. It was found that the initial velocity of the reaction (V0), maximal amount of the reaction product (Pmax) and characteristic time of the reaction (T) comprised 1.

[Identification of Ca2+ release channels in salivary glands secretory cells of Chironomus plumosus L].

The presence of two types of well-characterised Ca2+ release channels, namely IP3-receptors (Ins(1,4,5)P3Rs) and ryanodine-receptors (RyRs), was detected in the salivary glands secretory cells of Chironomus plumosus L. For this aim different blockators and activators of these Ca2+ -transport systems were used. The conditions for permeabilization of these cells by saponine were experimentally chosen for their more intensive action.