Deep mining of antibody phage-display selections using Oxford Nanopore Technologies and Dual Unique Molecular Identifiers.

Antibody phage-display technology identifies antibody-antigen interactions through multiple panning rounds, but traditional screening gives no information on enrichment or diversity throughout the process. This results in the loss of valuable binders. Next Generation Sequencing can overcome this problem.

Impact of minimally invasive surgery in the treatment of esophageal cancer.

Background: Surgical treatment of esophageal cancer is associated to a high morbidity and mortality rate. The open transthoracic or transhiatal esophagectomy are considerably invasive procedures and have been associated to high rates of complications and operative mortality. In this way, minimally invasive esophageal surgery has been suggested as an alternative to the classic procedures because would produce improvement in clinical longterm postoperative outcomes.

Recombinant chromosome 4 from a familial pericentric inversion: prenatal and adulthood wolf-hirschhorn phenotypes.

Pericentric inversion of chromosome 4 can give rise to recombinant chromosomes by duplication or deletion of 4p. We report on a familial case of Wolf-Hirschhorn Syndrome characterized by GTG-banding karyotypes, FISH, and array CGH analysis, caused by a recombinant chromosome 4 with terminal 4p16.3 deletion and terminal 4q35.

[Laparoscopic surgery for benign esophageal diseases].

Background: Minimally invasive surgery has the advantage of a lower rate of complications and can be used for benign esophageal diseases.

Aim: To report a single surgeon experience with laparoscopic surgery for benign esophageal diseases.

Material And Methods: Prospective analysis of 421 patients (160 males) with benign esophageal disease, who were subjected to laparoscopic surgery by a single surgeon.

[Primary esophageal motor disturbances among patients with esophageal symptoms].

Background: The esophagus can suffer several motor disturbances of striated or smooth muscle.

Aim: To determine the presence of primary motor disturbances of the esophagus among a group of patients with esophageal symptoms.

Material And Methods: Prospective study of 5,440 patients consulting for heartburn, chest pain or dysphagia, with primary esophageal motor disturbances, studied between 1994 and 2004.