Publications by authors named "M Ishinaga"

The contents of nitrate and nitrite in menus containing more than 350 g of vegetables per day were investigated. The amounts of vegetables in menu A developed by female students and menu B taken from a recipe book were 350+/-2 g and 457+/-77 g, respectively. The average content of the nitrate was 321.

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We developed a novel method that conveniently measures dietary choline-phospholipid content. Crude lipids extracted from dietary samples were reacted with phospholipase D from Streptomyces chromofuscus. The choline liberated from this reaction was then reacted with potassium triiodide, yielding choline periodide, which could be measured spectrophotometrically at 365 nm.

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The contents of nitrate and nitrite in vegetable juice (n = 63), tea beverage (n = 12) and mineral water (n = 15) were measured. The mean contents of nitrate were 0.73 +/- 14.

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Many dietary recommendations for the prevention of heart disease and hyperlipidemia include restriction of cholesterol intake. However, limiting cholesterol intake might also affect the intake of other nutrients. The daily intakes of cholesterol, lecithin, total fatty acids, and SFAs by 388 Japanese subjects (ages 6-59 y) were analyzed directly using the duplicate portion sampling technique.

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The changes in body fat ratio and serum lipids induced by the ingestion of beta-conglycinin were examined in 41 healthy female university student volunteers. The trend of change in body fat ratio following the ingestion of beta-conglycinin differed between students with a baseline body fat ratio over 25% and those less than 25%. In the former group, the ingestion of beta-conglycinin suppressed the increase in body fat ratio.

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