Immunofluorescence and biochemical investigation of the protective effects of naringin and diosmin on the freezability of merino ram semen.

Background: Various antioxidant substances are added to sperm extenders to protect spermatozoa against oxidative stress and cryodamage.

Objective: To investigate the effects of the flavonoid diosmin (DIO) and a flavanone glycoside naringin (NAR) on the freezability of ram semen.

Materials And Methods: In this study, six Merino rams were used during the breeding season.