Publications by authors named "M I Shikina"

To investigate the effects of growth (organ development) on tetrodotoxin (TTX) dynamics in the pufferfish body, TTX-containing feed homogenate was administered to 6- and 15-month old non-toxic cultured specimens of the pufferfish Takifugu rubripes at a dose of 40 mouse units (MU) (8.8 μg)/20 g body weight by oral gavage. After 24 h, the specimens were killed and the skin tissues (dorsal and ventral), muscle, liver, digestive tract, and gonads were separated.

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Article Synopsis
  • Research on the goby fish Yongeichthys criniger revealed that tetrodotoxin (TTX) distribution changes with maturation in female specimens collected over two time periods (2008-2010) in Okinawa, Japan.
  • The study identified different reproductive phases and found that TTX levels peaked in the ovary during the spawning period, suggesting that this fish accumulates TTX significantly in its reproductive organs.
  • Additionally, in a different group of specimens, TTX was mostly found in the skin during early maturation stages, but as the ovaries matured, TTX levels in the ovaries increased notably, especially in the later stages of yolk development.
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Microorganisms dwelling in drinking water reclaimed from water containing water in an enclosed environment were identified. The microbial species in the water reclaimed from the condensate of hydrogen peroxide decomposition products and from the air humidity condensate showed specific features and differences when compared to those in natural water.

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This paper presents data on the biological role of most macro- and trace elements contained in potable water and emphasizes the necessity of enriching it with mineral components. A profound study of the effect of water desalinated by various methods demonstrated that its salt composition should be standardized with respect to the optimal, allowable and threshold levels of mineralization. The dosage of salt and trace element supplements should be strictly controlled and therefore methods of artificial water mineralization should be thoroughly developed.

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In an enclosed environment the species composition of microorganisms was quantified and metabolic processes in microbial cells isolated from the atmospheric condensate and reclaimed water were investigated. It was demonstrated that indicator microorganisms isolated from water and condensate samples (Alcaligenes faecalis, Citrobacter freundii, Aeromonas hydrophila, Acinetobacter-Moraxella) were specific and differed from indicator microorganisms typical of regular tap water.

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