Co-infection of Lactococcus garvieae and Aeromonas hydrophila in cultured Nile Tilapia in Kerala, India.

Co-infection of Lactococcus garvieae and Aeromonas hydrophila, has been confirmed from diseased Nile Tilapia (Oreochromis niloticus), Chithralada strain cultured in a freshwater rearing pond of Alappuzha district of Kerala, India. The aetiological agents behind the disease outbreak were bacteriologically proven and confirmed by 16SrRNA sequencing and phylogenetic analysis. PCR detection of the virulent genes, showed existence of adhesin and hemolysin in L.

Morphological, biological and genomic characterization of lytic phages against Streptococcus agalactiae causing streptococcosis in tilapia.

Streptococcus agalactiae, a highly invasive pathogen causing streptococcosis, is a major disease imparting devastating effect in the aquaculture, worldwide. As bacteriophage therapy is getting more attention recently, as an alternative viable biocontrol agent to antibiotic and vaccine, this study aimed to isolate and purify obligately lytic bacteriophages and study its morphological, genetic and biological characteristics. Host range analysis of the four bacteriophages isolated in this study, such as Phage- 12 P, 15 F, 16 E and 20D exhibited 100% infectivity to S.

An improved method for efficient recovery of high quality DNA from date palm ( L; ).

Date palm ( L; ) is one of a few fruit trees that can remarkably grow in dessert agroecosystems that are characterized by extreme temperature fluctuations. Due to increasing demands for dates in the global market and commercial cultivation in many countries, the tree is currently under extensive research in many countries, particularly to improve the germplasm using different molecular tools. Most molecular techniques largely depend on good quality DNA in significant quantities, which are highly compromised by the presence of various contaminants in DNA.

Genomics, Molecular Epidemiology and Diagnostics of Infectious hypodermal and hematopoietic necrosis virus.

Infectious hypodermal and hematopoietic necrosis virus (IHHNV) is one of the major viral pathogens of penaeid shrimps worldwide, which has resulted in severe mortalities of up to 90 % in cultured Penaeus (Litopenaeus) stylirostris from Hawaii and hence designated Penaeus stylirostris densovirus (PstDNV). IHHNV is distributed in shrimp culture facilities worldwide. It causes large economic loss to the shrimp farming industry.

Molecular Biology and Epidemiology of Hepatopancreatic parvovirus of Penaeid Shrimp.

Hepatopancreatic parvovirus (HPV) is one of the major shrimp parvovirus which is known to cause slow growth in penaeid shrimps. HPV has been found in wild and cultured penaeid shrimps throughout the world and there is high genetic variation among the different geographic isolates/host species. Given its high prevalence, wide distribution and ability to cause considerable economic loss in shrimp aquaculture industry, HPV deserves more attention than it has received.