Publications by authors named "M I Prokofiev"

This article discusses the distinct characteristics of COVID-19 in pregnant women and investigates potential early predictors of disease severity in this specific patient population. The study included 116 pregnant women with a confirmed diagnosis of COVID-19 in different trimesters of pregnancy. In addition to clinical features, we evaluated general clinical research methods, biochemical parameters (procalcitonin, C-reactive protein, D-dimer), and the leukocyte index of endogenous intoxication and lymphocytic index to identify potential early predictors of disease severity.

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We studied the dynamics of in vitro maturation of bovine oocytes, the efficiency of asynchronously matured oocytes as recipients for the generation of embryos produced by nuclear transfer, and the potential for using blind enucleation of zona-free bovine oocytes in bovine cloning. At 15 h after the initiation of maturation (hpm), oocytes were freed from both cumulus cells and the zona pellucida, and the dynamics of oocyte maturation were monitored every 30 min through the criterion of extrusion of the first polar body (PB1). More than 41% of bovine oocytes had extruded PB1 by 16.

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Article Synopsis
  • The study evaluated how rabbit fibroblasts from different ages can be reprogrammed when using nuclear transfer (NT) to older oocytes.
  • The success rates of NT blastocysts were significantly higher when using G1 stage morula cells or fetal fibroblasts compared to younger and older fibroblast cells.
  • Additionally, serum starvation improved embryo development, leading to successful pregnancies with NT embryos, indicating that age and source of the fibroblast tissue affect reprogramming effectiveness during nuclear transfer.
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