[Expression of human interferon beta in the mammary gland of transgenic rabbits].

A biotechnological system for the production of human beta interferon was developed on the basis of a hybrid gene constructed from the coding sequence of the beta interferon gene inserted into the first exon of the sheep beta lactoglobulin gene. It is intended for the expression of human beta interferon in mammary glands of transgenic animals. Two lines of transgenic rabbits were obtained using the hybrid gene.

[Efficacy of various methods of a reporter gene transfer to chicken embryonic cells].

The methods of transfection ofa plasmid with a reporter gene involving DNA injection into chicken embryonic cells were studied. The parameters of the efficient transfection of chicken blastodermal cells with a foreign gene have been determined (20-24 and up to 40% in culture and embryos, respectively). A high efficiency of transfection of primordial germ cells isolated from the gonads has been obtained after DNA injection into the dorsal aorta of 2.

[The course of the nearest postoperative period in patients with injuries of the spleen].

The authors have analyzed the course of the postoperative period in 410 patients operated on for isolated, combined and multiple traumas with injuries of the spleen. The character of the operation (splenectomy, splenectomy with autolientransplantation, organ preserving operations) had no significant influence on frequency of complications or lethality.

[Production of cloned bovine embryos by somatic cell transfer into enucleated zona-free oocytes].

Cloned bovine embryos were produced at the blastocyst stage. Prior to enucleation, oocytes were freed from the zona pellucida. Fibroblasts isolated from the bovine fetus were used as nuclear donors.

[A study of factors affecting the efficiency of electrofusion of enucleated eggs and cell-donors of nuclei].

We studied the capacity of rabbit oocytes for electrofusion with morula blastomeres and fetal fibroblasts. The morula blastomeres fused with aging ooplasts more readily than the fetal fibroblasts: 92.9 versus 63.