Anaphylaxis inhibitory factor in IgE-dependent mast cell stimulation.

We have previously demonstrated that there is a factor present in some human serum which inhibits the passive cutaneous anaphylaxis reaction mediated by IgE. The present study analyzes the effect of this factor on mast cell IgE-dependent tumor necrosis factor (TNF)-alpha release. Rat peritoneal mast cells and RBL-2H3 cells treated with monoclonal mouse IgE anti-dinitrophenol (anti-DNP) followed by DNP-bovine serum albumin (DNP-BSA) were used and TNF-alpha release was measured at different time points.

An anaphylaxis inhibitory factor present in mouse serum.

We have previously described that normal mouse serum inhibits the PCA reaction mediated by IgE. The present study attempts to characterize this PCA inhibitory factor from the biologically active fraction of the serum. The physicochemical properties of this glycoprotein are the following: it is inactivated at 55 degrees C; it has a molecular weight between 182 kD and 240 kD, determined by gel filtration; it shows affinity to concanavalin A and lentil lectin but not to peanut agglutin; it demonstrates affinity to IgE and, apparently, its carbohydrate moiety is not required for its biological activity.

Suppression of passive cutaneous anaphylactic reaction by a human serum factor.

The capacity of human serum to inhibit the IgE-mediated passive cutaneous anaphylactic reaction in mouse was established. The active fraction was isolated by ammonium sulfate precipitation (33-40% saturation). Its molecular weight, as estimated by gel filtration, was found to be 42-66 kd.

Anaphylaxis inhibitory factor: clinical evaluation in pediatric patients.

A factor similar to that reported in mouse, capable of inhibiting the passive cutaneous anaphylactic reaction (PCA), has been demonstrated in human sera. The present study reports the laboratory and clinical evaluation of this factor in pediatric patients. A low percentage of the allergic patients (7.

Sex modifies IgE antibody response.

In the present work the IgE response between male and female mice was compared. Normal and castrated RF mice of both sexes were immunized with 100 micrograms OA in 20 mg aluminum hydroxide gel. Results show that IgE response is lower in male than in female mice when assessed by the PCA test.