Clinicopathologically Defined Nevus Subtypes and Melanoma Risk.

Early detection of melanoma is a major determinant in disease outcome and drives the number of (over)excised nevi in clinical practice. This study aimed to evaluate demographic features and melanoma risk of clinically suspicious, mainly flat nevus subtypes. Based on the methodology of ex vivo dermoscopy and derm dotting, the 12 most prevalent nevus subtypes were identified in a collection of over 7000 nevi excised for medical reasons.

Computed tomographic evaluation of the proximity of needles placed for perineural anesthesia of the palmar digital nerves to synovial structures in the foot: an study.

Background: Potential synovial penetration following palmar digital nerve blocks has not been investigated.

Objectives: To evaluate the proximity of needles placed for palmar digital nerve blocks to nearby synovial structures using computed tomography (CT).

Study Design: Descriptive observational study.

Longitudinal pilot study examining the effect of punch biopsy on equine sarcoid growth dynamics.

Background: Nonexcisional tissue biopsies facilitate pre-operative confirmation of equine sarcoid yet fear of lesion deterioration currently limits its use in the diagnostic workup.

Objectives: To evaluate the effect of a single punch biopsy on tumour growth dynamics [thickness, area, circumference, viral load (VL) and Visual Analog Scale (VAS)].

Animals: Six client-owned horses with 11 sarcoids of various classification.

Dobutamine use in horses during romifidine and isoflurane anaesthesia.

This retrospective study aimed to assess the incidence of hypotension and the subsequent administration of dobutamine in horses anesthetized with isoflurane and romifidine during elective surgery. Time from induction of anaesthesia to administration of dobutamine was registered, as well as the time and dose needed to restore mean arterial pressure (MAP) ≥ 70 mmHg. Additionally, the influence of patient and anaesthesia related parameters on the need for dobutamine supplementation was evaluated.

CRISPR/Cas9-Mediated Targeting of BPV-1-Transformed Primary Equine Sarcoid Fibroblasts.

Equine sarcoids (EqS) are fibroblast-derived skin tumors associated with bovine papillomavirus 1 and 2 (BPV-1 and -2). Based on Southern blotting, the BPV-1 genome was not found to be integrated in the host cell genome, suggesting that EqS pathogenesis does not result from insertional mutagenesis. Hence, CRISPR/Cas9 implies an interesting tool for selectively targeting BPV-1 episomes or genetically anchored suspected host factors.