Publications by authors named "M Golzio"
Article Synopsis
- Bacterial resistance is increasing, necessitating innovative strategies for effective antibiotic treatment.
- Ultra-short propelling magnetic nanochains, designed to mimic natural bacterial movement, enhance the efficacy of antibiotics against biofilm-forming Staphylococcus epidermidis by converting resistant bacteria into sensitive ones.
- These nanochains, activated by a low-intensity rotating magnetic field, operate by mechanically disrupting bacterial structures and working synergistically with antibiotics to completely eliminate biofilms.
The understanding of the mechanisms involved in DNA electrotransfer in human skin remains modest and limits the clinical development of various biomedical applications, such as DNA vaccination. To elucidate some mechanisms of DNA transfer in the skin following electroporation, we created a model of the dermis using a tissue engineering approach. This model allowed us to study the electrotransfection of fibroblasts in a three-dimensional environment that included multiple layers of fibroblasts as well as the self-secreted collagen matrix.
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- Skin electroporation uses Pulsed Electric Fields (PEFs) to temporarily disrupt the skin barrier, enhancing non-invasive drug delivery compared to traditional methods like injections.
- A drug delivery system has been developed that combines hydrogels as drug reservoirs and electrodes for applying electrical pulses on the skin.
- The study employed a mouse skin model and revealed that PEFs at specific voltages increased drug uptake and enhanced skin conductivity, demonstrating two key electroporation phases: initial transport in skin lipids at 100 V and cell permeabilization at 300 V.
Cancer Imaging by Intravital Microscopy: The Dorsal Window Chamber Model.
Methods Mol Biol
January 2024
Article Synopsis
- Intravital microscopy enables real-time observation of cell behavior within a living organism's complex environment.
- This technique allows for longitudinal studies, tracking structural and functional changes over extended periods in the same animal.
- In cancer research, the dorsal window chamber model is preferred for studying tumor-related processes like cell migration and interactions between cells and blood vessels, using advanced microscopy methods for detailed analysis.