Escherichia coli and Micrococcus luteus Activate the CG45045 Gene in Drosophila S2 Cell Line.

The humoral immune system of Drosophila melanogaster, which is the best studied of all eukaryotes, is activated by the canonical IMD and Toll signalling pathways. Recently, long non-coding RNAs (lncRNAs) and genes encoding short polypeptides have been identified as potential regulators of the innate immune response. S2 cells are a macrophage-like cell line.

Ecdysone promotes gene- and pathogen-specific immune responses to Micrococcus luteus and Bacillus subtilis in Drosophila S2 cells.

In Drosophila, the 20-hydroxyecdysone (20E) hormone regulates numerous essential biological processes. Here, we studied the contribution of 20E to the activity of immune signaling pathways and antimicrobial activity using the model Drosophila S2 cells. We found that while 20E alone has no essential effect on this system, pretreating S2 cells with 20E followed by incubation with Escherichia coli or Micrococcus luteus stimulates the induction of a limited number of antimicrobial peptide (AMP) genes, such as Diptericin (Dpt) and Drosomycin (Drs).

Early ultrastructural lesions of apoptosis induced in vivo in two varieties of tissues (thymus and kidneys) after a single whole-body-gamma irradiation of adult mice.

Three groups of adult Swiss albino female mice with an average body weight of 20 grams were exposed to a whole-body irradiation by 137-cesium gamma rays at 2, 4 and 6 Gy (1.4 Gy/min.).