Publications by authors named "M Gharakhani"
Article Synopsis
- The study focuses on cloning and sequencing a gene related to a protein that is preserved across different pathogenic serovars during infections.
- 23 pathogenic and 2 non-pathogenic serovars were analyzed, sourced from a microbiology lab in Iran, with three serovars also used in a trivalent vaccine.
- The results showed that the gene was present in all pathogenic serovars but absent in non-pathogenic ones, highlighting its potential for creating an effective recombinant vaccine due to its high conservation (95.5% to 100% similarity).
Leptospirosis is a worldwide zoonotic disease caused by pathogenic spp, often occurring in tropical and subtropical regions. Focusing on development of rapid diagnostic methods to facilitate early diagnosis and a universal vaccine are the main critical issues to overcome the burden of leptospirosis. Here, we have studied the immunogenic potential of prepared recombinant Loa22 protein (rLoa22) of local pathogenic species in mice and its ability to induce humoral and cellular immunity, being further evaluated by analyzing the immunoglobulin G (IgG) subclasses and cytokines produced through immunization.
View Article and Find Full Text PDFBackground And Objectives: Leptospirosis is a zoonotic disease caused by pathogenic serovars. The genus cannot differentiated by conventional techniques. However, identity determination of pathogenic serovar is precious of public health problems and epidemiological studies.
View Article and Find Full Text PDFAlthough, strengthening patient safety competencies in nursing has been emphasized for enhancing quality care and patient safety. However, little is known about the association of nurses' perceptions of patient safety competency with adverse nurse outcomes in Iranian hospitals. This study aimed to measure nurses' levels of patient safety competency in the hospitals of Iran and examines the relationship between patient safety competency with the occurrence and reporting of adverse events (AEs).
View Article and Find Full Text PDFBackground And Objectives: One of the highly conserved outer membrane proteins expressed only by pathogenic Leptospires is Loa22. The study aims is to achieve the optimum conditions for high expression of recombinant Loa22 (rLoa22) protein.
Materials And Methods: Complete coding sequence of gene sub-cloned into a pET32a (+) expression vector.