Yeast perilipin Pet10p/Pln1p interacts with Erg6p in ergosterol metabolism.

Lipid droplets (LD) are highly dynamic organelles specialized for the regulation of energy storage and cellular homeostasis. LD consist of a neutral lipid core surrounded by a phospholipid monolayer membrane with embedded proteins, most of which are involved in lipid homeostasis. In this study, we focused on one of the major LD proteins, sterol C24-methyltransferase, encoded by ERG6.

Comparative Analysis of Two Isolates Originating from the Same Patient Harbouring the Y132F and R398I Mutations in the Gene.

This work presents a comparative analysis of two clinical isolates of , isolated from haemoculture (HC) and central venous catheter (CVC). Both strains harboured Y132F and R398I mutations in the gene associated with resistance to fluconazole (FLC). Differences between the HC and CVC isolates were addressed in terms of virulence, resistance to FLC, and lipid distribution.

Heterologous Production of Calendic Acid Naturally Found in by Recombinant Fission Yeast.

Calendic acid (CA) is a conjugated fatty acid with anti-cancer properties that is widely present in seed oil of . Using the co-expression of fatty acid conjugases (CoFADX-1 or CoFADX-2) and fatty acid desaturase (PgFAD2), we metabolically engineered the synthesis of CA in the yeast without the need for linoleic acid (LA) supplementation. The highest CA titer and achieved accumulation were 4.

Squalene lipotoxicity in a lipid droplet-less yeast mutant is linked to plasma membrane dysfunction.

Squalene is a naturally occurring triterpene with wide industrial applications. Due to limited natural resources, production of this valuable lipid in yeast is of high commercial relevance. Typically low levels of squalene in yeast can be significantly increased by specific cultivation conditions or genetic modifications.