[The RH-PA cell line--a source for obtaining the urokinase-type plasminogen activator].

A new cell line (RH-PA) was established on the basis of the human embryonic kidney cell line (RH). The new line produces urokinase type plasminogen activator (PA). The activity of the activator amounted to 150-200 IU/ml estimated with the procedure of fibrin plates.

[A study of the cloned human erythropoietin gene expression in mammalian cells in vitro].

The human gene coding for the principal factor of erythroid cells differentiation, erythropoietin, has been isolated from the genomic phage library using an oligonucleotide probe for the gene. The construction of series of plasmids carrying the erythropoietin gene under the control of various regulatory elements is reported. Efficiency of the erythropoietin gene expression was estimated by testing of the biologically active erythropoietin in conditioned media 48 h after transient transfection of COS 1 and CHO cell lines.