[Interneurons Brainstem of the Human].

Objective: To conduct a comparative study of the structural organization of internuclear interneurons involved in the metabolism of nitrogen monoxide, hydrogen sulphide and carbon monoxide in the caudal brain stem humans.

Methods: The cross-sectional study was performed. We used histochemical and immunohistochemical methods to study the internuclear interneurons expressing neuronal nitric oxide synthase, cystathionine β-synthase and heme oxygenase-2 which are located between the giant and small cell reticular nuclei (cell group 1), small cell reticular nucleus and the nucleus of the solitary tract (cell group 2) or is surrounded by reticular lateral nucleus (cell group 3).

Topochemistry of Internuclear and Intranuclear Interneurons of the Vasomotor Area in the Medulla Oblongata of Hypertensive Rats.

Immunohistochemical examination with the antiserum against neuronal NO synthase and cystathionine β-synthase was used to study the following two pools of interneurons in Wistar rats at various periods after the development of renovascular hypertension: intranuclear interneurons (lying in the projection of the solitary nucleus, reticular gigantocellular nucleus, and parvocellular nucleus) and 2 groups of internuclear interneurons (small interneurons, area 50-300 μ(2); and large interneurons, area above 350 μ(2)). Intranuclear and internuclear interneurons probably play a role in the central mechanisms of hemodynamics regulation. These interneurons differ by not only in topochemical parameters, but also functional properties (different resistances to BP changes).

[POSSIBILITIES OF THE FAT TISSUE TRANSPLANTATION FOR THE FACE ATROPHY OF VARIOUS ETIOLOGY].

Experience of transplantation of the fat tissue (FT, lipofilling) in 4 patients, treated for facial atrophy of various origin, was analyzed. Pathological states in the patients have included facial inborn hemiatrophy, posttraumatic atrophy in conjunction with the bone sceleton integrity disorder, cicatricial and iatrogenic atrophy due to earlier performed operation. In all the patients the fat transplant preparation technology, using method of manual lipoaspiration and centrifugation, was applied.

Two pools of interneurons in the bulbar region of the cardiovascular center of rats.

Wistar rats were subjected to renovascular hypertension. Then, we studied two pools of neurons: intranuclear neurons located in projection area of the solitary tract nucleus and reticular, magnocellular, and parvicellular nuclei; and two groups of internuclear neurons, which included both small neurons 50-300 µm(2) in size and large neurons more than 350 µm(2) in size. Immunohistochemical methods with antisera against neuronal nitric oxide synthase, cystathionine-β-synthase, and heme oxygenase-2 were used.

[Topochemistry of internuclear interneurons in the human brain stem in arterial hypertension].

Objective: To study the internuclear interneurons (IN) involved in the metabolism of nitric oxide, hydrogen sulfide and carbon monoxide in the caudal brainstem in healthy people and those with lifetime hypertension.

Material And Methods: The study was performed on postmortem material of 8 healthy men and 19 men, aged 18-44 years, with lifetime arterial hypertension. Immunohistochemistry methods for nNOS, HO-2, CBS and histochemical method for NADPH-diaphorase were used to study IN groups located between the giant and small cell reticular nuclei (IN1), small cell reticular nucleus and the nucleus of the solitary tract (IN2) surrounded by the lateral reticular nucleus (IN3).