Preventive effects of thymoquinone on glyco-nitro-oxidized human fibrinogen: A comprehensive biophysical study projecting possible therapeutic role in diabetes and associated complications.

Persistence of long-term hyperglycemia results in the glyco-oxidation of plasma proteins, which is considered to be a significant factor in metabolic dysfunction, linking hyperglycemia to the emergence of vascular complications. Methylglyoxal (MGO), a dicarbonyl species formed excessively under diabetes, elevates the oxidative stress, enhancing the generation of superoxide anion, which ultimately reacts with nitric oxide (NO•) to form peroxynitrite (PON). PON, being a powerful nitro-oxidizing agent distorts protein structure, hampering its function.

Grade-ADOLOPMENT of nephrology clinical practice guidelines and creation of referral algorithms for Pakistan.

Objective: To develop evidence-based local clinical practice guidelines and primary care referral pathways for general physicians to help streamline the management of glomerular diseases and diabetes in chronic kidney disease patients in Pakistan.

Methods: The study was conducted from October 2021 to February 2023 at the Centre for Clinical Best Practices, Aga Khan University Hospital, Karachi, in collaboration with the AKUH Section of Nephrology. Two source guidelines were selected by the local nephrologists after a thorough literature review on PubMed and Google Scholar.

Angiotensin-Converting Enzyme 2 (G8790A) Gene Polymorphism as a Risk Factor for COVID-19 in Egyptian Children and Adolescents.

Objective: Recently, angiotensin-converting enzyme 2 (ACE2) gene has emerged as a potential candidate gene for susceptibility to SARS-CoV-2 infection. We investigated whether ACE2 G8790A (rs2285666) polymorphism could be a genetic marker for susceptibility to COVID-19 and disease severity in Egyptian children and adolescents.

Methods: This was a prospective case-control study included 580 cases diagnosed with COVID-19, and 580 matched control children and adolescents.