Publications by authors named "M Fukuhara"
Adeno-associated virus (AAV) vectors have attracted significant attention as the main platform for gene therapy. To ensure the safety and efficacy of AAV vectors when used as gene therapy drugs, it is essential to assess their critical quality attributes (CQAs). These CQAs include the genome packaging status, the size of the genome encapsidated within the AAV capsid, and the stoichiometry of viral proteins (VPs) that constitute the AAV capsids.
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- This study compares two endoscopic techniques, endoscopic submucosal dissection (ESD) and endoscopic laryngo-pharyngeal surgery (ELPS), for treating superficial pharyngeal carcinomas to assess their effectiveness.
- A review of 93 patients showed that ELPS had significantly shorter procedure times and led to less laryngeal edema compared to ESD, although long-term survival outcomes were similar for both methods.
- The findings suggest that while ELPS is quicker and less damaging, it has limitations in treating narrow areas; therefore, combining ELPS with ESD may enhance treatment efficacy.
Hemodialysis therapy is an extracorporeal circulation treatment that serves as a substitute for renal function. In Japan, patients receive this efficient four-hour treatment, three times per week, allowing them to maintain a social life nearly equivalent to that of healthy individuals. Before the treatment, two punctures are performed to establish extracorporeal circulation, and a high blood flow rate is essential to ensure efficient therapy.
View Article and Find Full Text PDFAim: Malnutrition is a prevalent health issue among hemodialysis patients. Oral frailty, a condition characterized by impairments in multiple oral health aspects and functions, has been associated with nutritional status in the general population. We aimed to determine whether oral frailty was associated with nutritional status in hemodialysis patients aged ≥50 years.
View Article and Find Full Text PDFThe adeno-associated virus (AAV) vector is one of the most advanced platforms for gene therapy because of its low immunogenicity and non-pathogenicity. The concentrations of both AAV vector empty particles, which do not contain DNA and do not show any efficacy, and AAV vector full particles (FPs), which contain DNA, are important quality attributes. In this study, a dual fluorescence-linked immunosorbent assay (dFLISA), which uses two fluorescent dyes to quantify capsid and genome titers in a single analysis, was established.
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