Toward a comprehensive profiling of alternative splicing proteoform structures, interactions and functions.

The mRNA splicing machinery has been estimated to generate 100,000 known protein-coding transcripts for 20,000 human genes (Ensembl, Sept. 2024). However, this set is expanding with the massive and rapidly growing data coming from high-throughput technologies, particularly single-cell and long-read sequencing.

Stability of [10-12]cycloparaphenylene complexes with pristine fullerenes C and endohedral metallofullerenes MN@C.

[]Cycloparaphenylenes ([]CPPs) are strained macrocycles, comprising only sp-hybridized carbon atoms. In recent years, []CPPs have become of great research interest in the field of supramolecular chemistry since their special structure enables the formation of novel host-guest complexes. In this work, we investigate the gas-phase chemistry of noncovalent complexes of [10-12]CPP with the pristine fullerenes C and the endohedral metallofullerenes (EMFs) ScN@-C, ScN@-C and MN@-C (M = Sc, Y, Lu, Gd).

Nanohoops Favour Light-Induced Energy Transfer over Charge Separation in Porphyrin/[10]CPP/Fullerene Rotaxanes.

[2]Rotaxanes offer unique opportunities for studying and modulating charge separation and energy transfer, because the mechanical bond allows the robust, yet spatially dynamic tethering of photoactive groups. In this work, we synthesized [2]rotaxane triads comprising a central (aza)[10]CPP⊃C bis-adduct complex and two zinc porphyrin stoppers to address how the movable nanohoop affects light-induced charge separation and energy transfer between the rotaxane subcomponents. We found that neither the parent nanohoop [10]CPP nor its electron-deficient analogue aza[10]CPP actively participate in charge separation.

Predicting the Dynamic Interaction of Intrinsically Disordered Proteins.

Intrinsically disordered proteins (IDPs) participate in various biological processes. Interactions involving IDPs are usually dynamic and are affected by their inherent conformation fluctuations. Comprehensive characterization of these interactions based on current techniques is challenging.